| Kode Mata Kuliah | BM2203 / 2 SKS |
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| Penyelenggara | 104 - Mikrobiologi / SITH |
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| Kategori | Kuliah |
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| Bahasa Indonesia | English |
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| Nama Mata Kuliah | Proyek Biologi Sel Molekuler dan Genetika Mikroba | Molecular Cell Biology and Microbial Genetics Project |
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| Bahan Kajian | - Pembuatan larutan analisis molekuler dan genetika mikroba
- Snapgene dan benchling
- Isolasi DNA dan RNA
- Sintesis cDNA dan Polymerase Chain Reaction (PCR)
- Purifikasi Hasil PCR, Ligasi, dan Pembuatan Sel Kompeten
- qPCR dan analisis hasil
- Transformasi, spreading, dan blue-white screening
- PCR Koloni, isolasi plasmid, dan restriksi
- Analisis hasil Sanger sequencing
- Kontrol Produksi, Isolasi, Purifikasi, dan Visualisasi Protein Rekombinan
- Knock-out gen pada Escherichia coli dengan teknik recombineering
- Pengenalan berbagai jenis sel
- Mitosis Sel dan Efek Sitogenik Logam Terhadap Nilai Indeks Mitosis
- Kromosom Politen pada Drosophila melanogaster
| - Preparation of molecular biology and microbial genetics solutions
- Introduction to SnapGene and Benchling software
- DNA and RNA isolation
- cDNA synthesis and Polymerase Chain Reaction (PCR)
- PCR product purification, ligation, and preparation of competent cells
- Quantitative PCR (qPCR) and data analysis
- Transformation, spreading, and blue-white screening
- Colony PCR, plasmid isolation, and restriction analysis
- Sanger sequencing data analysis
- Protein production control, isolation, purification, and visualization
- Gene knock-out in Escherichia coli using recombineering techniques
- Introduction to different cell types
- Cell mitosis and cytogenetic effects of metals
- Polytene chromosome analysis in Drosophila melanogaster
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| Capaian Pembelajaran Mata Kuliah (CPMK) | - Mampu menerapkan metode yang berkaitan dengan konsep mikrobiologi dan mengoperasikan peralatan standar laboratorium mikrobiologi. (CPL 09)
- Mampu menerapkan metode yang berkaitan dengan konsep biologi molekuler dan mengoperasikan peralatan standar laboratorium biologi molekuler. (CPL 10)
- Mampu mengaplikasikan prinsip keselamatan kerja dengan menggunakan peralatan perlindungan dan menerapkan prosedur darurat yang sesuai. (CPL 11)
| - Students are able to apply methods related to microbiology concepts and laboratory techniques.
- Students are able to apply methods related to molecular biology concepts and laboratory techniques.
- Students are able to apply laboratory safety principles and procedures.
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| Metode Pembelajaran | Praktikum | Laboratory practical sessions |
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| Modalitas Pembelajaran | Praktikum langsung yang dibimbing oleh asisten dan pimpinan praktikum; Praktikum mandiri terarah | Supervised hands-on laboratory practical sessions |
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| Jenis Nilai | ABCDE |
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| Metode Penilaian | 35% UTS, 35% UAS, 30% Tugas (penilaian akhir laporan) | Midterm Exam 35%, Final Exam 35%, Assignments 30% (final report assessment) |
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| Catatan Tambahan | | |
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