Mata Kuliah BM2203 - 2024

Kode Mata KuliahBM2203 / 2 SKS
Penyelenggara104 - Mikrobiologi / SITH
KategoriKuliah
Bahasa IndonesiaEnglish
Nama Mata KuliahProyek Biologi Sel Molekuler dan Genetika MikrobaMolecular Cell Biology and Microbial Genetics Project
Bahan Kajian
  1. Pembuatan larutan analisis molekuler dan genetika mikroba
  2. Snapgene dan benchling
  3. Isolasi DNA dan RNA
  4. Sintesis cDNA dan Polymerase Chain Reaction (PCR)
  5. Purifikasi Hasil PCR, Ligasi, dan Pembuatan Sel Kompeten
  6. qPCR dan analisis hasil
  7. Transformasi, spreading, dan blue-white screening
  8. PCR Koloni, isolasi plasmid, dan restriksi
  9. Analisis hasil Sanger sequencing
  10. Kontrol Produksi, Isolasi, Purifikasi, dan Visualisasi Protein Rekombinan
  11. Knock-out gen pada Escherichia coli dengan teknik recombineering
  12. Pengenalan berbagai jenis sel
  13. Mitosis Sel dan Efek Sitogenik Logam Terhadap Nilai Indeks Mitosis
  14. Kromosom Politen pada Drosophila melanogaster
  1. Preparation of molecular biology and microbial genetics solutions
  2. Introduction to SnapGene and Benchling software
  3. DNA and RNA isolation
  4. cDNA synthesis and Polymerase Chain Reaction (PCR)
  5. PCR product purification, ligation, and preparation of competent cells
  6. Quantitative PCR (qPCR) and data analysis
  7. Transformation, spreading, and blue-white screening
  8. Colony PCR, plasmid isolation, and restriction analysis
  9. Sanger sequencing data analysis
  10. Protein production control, isolation, purification, and visualization
  11. Gene knock-out in Escherichia coli using recombineering techniques
  12. Introduction to different cell types
  13. Cell mitosis and cytogenetic effects of metals
  14. Polytene chromosome analysis in Drosophila melanogaster
Capaian Pembelajaran Mata Kuliah (CPMK)
  1. Mahasiswa mampu melakukan isolasi dan analisis kualitas DNA dan/atau RNA. (CPL 09)
  2. Mahasiswa mampu merancang strategi amplifikasi nukleotida sesuai dengan tujuan yang ditentukan. (CPL 09)
  3. Mahasiswa mampu merancang strategi kloning dan melakukannya, baik untuk tujuan perbanyakan maupun rekayasa protein. (CPL 09)
  4. Mahasiswa mampu menerapkan prinsip keselamatan kerja, biosafety, dan biosecurity selama proyek, termasuk penggunaan APD dan biosafety cabinet, penanganan bahan berbahaya, pengelolaan limbah biologis dan organisme hasil rekayasa genetika, serta pelaksanaan prosedur darurat. (CPL 09)
  1. Students are able to perform isolation and quality analysis of DNA and/or RNA. (PLO 9)
  2. Students are able to design nucleotide amplification strategies in accordance with specified objectives. (PLO 9)
  3. Students are able to design and execute cloning strategies for both amplification and protein engineering purposes. (PLO 9)
  4. Students are able to apply the principles of occupational safety, biosafety, and biosecurity during the project, including the use of PPE and biosafety cabinets, handling of hazardous materials, management of biological waste and genetically modified organisms, as well as the execution of emergency procedures. (PLO 9)
Metode PembelajaranPraktikumLaboratory practical sessions
Modalitas PembelajaranPraktikum langsung yang dibimbing oleh asisten dan pimpinan praktikum; Praktikum mandiri terarahSupervised hands-on laboratory practical sessions
Jenis NilaiABCDE
Metode Penilaian35% UTS, 35% UAS, 30% Tugas (penilaian akhir laporan)Midterm Exam 35%, Final Exam 35%, Assignments 30% (final report assessment)
Catatan Tambahan