Mata Kuliah KI3262 - 2024

Kode Mata KuliahKI3262 / 1 SKS
Penyelenggara105 - Kimia / FMIPA
KategoriKuliah
Bahasa IndonesiaEnglish
Nama Mata KuliahPraktikum Metabolisme dan Informasi GenetikaMetabolism and Genetic Information Practicum
Bahan Kajian
  1. Teknik dasar mikrobiologi: cara penumbuhan mikroba dan pekerjaan aseptik.
  2. Metabolisme asam amino dan CO2: pengukuran aktivitas enzim pada jalur metabolisme manusia dan pengukuran laju fotosintesis pada mikroalga hijau.
  3. Pengenalan teknik genetika molekuler: PCR dan aplikasinya untuk identifikasi mikroba prokariot dan eukariot.
  4. Regulasi dan ekspresi gen: sistem Lac Operon
  5. Teknik Bioinformatika: BLAST, DNA alignment dan analisis jalur metabolisme.
  1. Basic microbiology techniques: how to grow microbes and aseptic work.
  2. Amino acid and CO2 metabolism: measurement of enzyme activity in human metabolic pathways and measurement of photosynthesis rates in green microalgae.
  3. Introduction to molecular genetic techniques: PCR and its application for the identification of prokaryotic and eukaryotic microbes.
  4. Gene regulation and expression: the Lac Operon system
  5. Bioinformatics techniques: BLAST, DNA alignment and metabolic pathway analysis.
Capaian Pembelajaran Mata Kuliah (CPMK)
  1. Mahasiswa menerapkan prinsip pengukuran aktivitas enzim untuk mengukur aktivitas enzim transaminase glutamat-piruvat yang terlibat dalam metabolosme asam amino dari sampel serum darah.
  2. Mahasiswa menjelaskan keterkaitan reaksi terang dan asimilasi CO₂ berdasarkan data laju fotosintesis yang diukur secara eksperimental
  3. Mahasiswa menerapkan prinsip dan teknik dasar genetika molekuler (PCR, elektroforesis, sekuensing) untuk mengamplifikasi dan menganalisis gen pengode 16S rRNA bakteri.
  4. Mahasiswa menerapkan prinsip dasar regulasi dan ekspresi gen serta metode laboratorium terkait untuk menganalisis aktivitas β-galaktosidase pada sistem Lac Operon.
  5. Mahasiswa menggunakan software dasar berbasis web untuk menganalisis biomolekul, contohnya DNA.
  6. Mahasiswa mampu menjawab tugas pendahuluan praktikum dengan informasi dari buku teks dan artikel ilmiah.
  7. Mahasiswa mengolah informasi/data dari buku teks dan/atau artikel ilmiah untuk menganalisis data percobaan dan menyusun laporan praktikum.
  8. Mahasiswa menerapkan informasi MSDS bahan kimia untuk mengidentifikasi bahaya dan menentukan tindakan pencegahan yang sesuai dalam kegiatan praktikum.
  9. Mahasiswa mengaplikasikan prinsip biosafety dengan menggunakan alat pelindung diri (jas lab, sarung tangan, kacamata pelindung) secara disiplin saat bekerja di laboratorium.
  10. Mahasiswa mengoperasikan peralatan praktikum (spektrofotometer, microplate reader, mesin PCR, elektroda Clark, elektroforesis agarosa) sesuai prosedur.
  11. Mahasiswa menulis laporan praktikum yang mencakup teori dasar, metode, hasil, dan analisis hasil eksperimen secara logis dan terstruktur.
  12. Mahasiswa menyusun materi presentasi ilmiah yang baik dan benar berdasarkan data hasil praktikum.
  13. Mahasiswa mempresentasikan hasil praktikum secara lisan dalam seminar akhir praktikum.
  14. Mahasiswa berpartisipasi aktif dalam kegiatan praktikum kelompok dan menyelesaikan tugas kelompok sesuai pembagian kerja dan tenggat waktu yang disepakati.
  15. Mahasiswa bekerja sama dalam kelompok praktikum dengan menunjukkan sikap terbuka, objektif, dan proaktif sehingga memperoleh penilaian kinerja yang baik dari anggota kelompok.
  16. Mahasiswa menggunakan software bioinformatika (BLAST, Clustal Omega) untuk mengolah data biomolekul dan mengidentifikasi spesies bakteri.
  1. Students applied the principles of enzyme activity measurement to measure the activity of glutamate-pyruvate transaminase, an enzyme involved in amino acid metabolism, in blood serum samples.
  2. The student explains the relationship between the light reaction and CO₂ assimilation using data on the photosynthesis rate obtained through experimentation.
  3. Students will use fundamental molecular genetic principles and techniques, including PCR, electrophoresis and sequencing, to amplify and analyse bacterial 16S ribosomal RNA (rRNA) genes.
  4. Students apply the fundamental principles of gene regulation and expression, as well as the relevant laboratory methods, to analyse β-galactosidase activity in the Lac operon system.
  5. Students use basic web-based software to analyse biomolecules, such as DNA.
  6. Students are able to answer preliminary practical assignments with information from textbooks and scientific articles.
  7. Students are able to select and process information/data from textbooks and/or scientific articles for analyzing practical experiment data and writing practical reports.
  8. Students use the information in the chemical MSDS to identify hazards and determine appropriate precautionary measures during practical sessions.
  9. Students apply biosafety principles by consistently wearing personal protective equipment (lab coats, gloves, safety goggles) whilst working in the laboratory. Students apply biosafety principles by consistently wearing personal protective equipment (lab coats, gloves, safety goggles) whilst working in the laboratory.
  10. Students operate the laboratory equipment (spectrophotometer, microplate reader, PCR machine, Clark electrode, agarose electrophoresis) in accordance with the procedures.
  11. Students write laboratory reports covering the basic theory, methods, results and analysis of the experimental results in a logical and structured manner.
  12. Students prepare well-structured and accurate scientific presentation materials based on data from their practical work.
  13. Students present the results of their practical work orally at the final practical seminar.
  14. Students actively participate in group practical sessions and complete group assignments in accordance with the agreed division of labour and deadlines.
  15. Students work together in practical groups, demonstrating an open, objective and proactive attitude, thereby receiving positive performance assessments from their group members.
  16. Students use bioinformatics software (BLAST, Clustal Omega) to process biomolecular data and identify bacterial species.
Metode PembelajaranCeramah, diskusi kelompok, presentasi, praktikum, penulisan laporanLectures, group discussions, presentations, practicums, report writing
Modalitas PembelajaranAudio,visual, kinestetika, sinkronousAudio, visual, kinesthetic, synchronous
Jenis NilaiABCDE
Metode PenilaianTes harian, keterampilan kerja, laporan, UTS, UAS, tugas presentasiDaily tests, work skills, reports, midterm exams, final exams, presentation assignments
Catatan Tambahan