| Bahan Kajian | - Interaksi intra dan intermolekul dalam biomolekul
- Struktur primer protein & asam nukleat
- Struktur sekunder protein & asam nukleat
- Transisi coil-heliks
- Struktur 3D protein & asam nukleat
- Fungsi protein: energetika katalisis enzim
- Fungsi protein: energetika proses transport via membran
- Model Folding protein
- Termodinamika folding protein
- Kinetika folding protein
- Interaksi antar makromolekul
- Interaksi non-kooperatif makromolekul-ligan
- Interaksi kooperatif makromolekul-ligan
| - Intra- and Intermolecular Interactions in Biomolecules
- Primary Structure of Proteins & Nucleic Acids
- Secondary Structure of Proteins & Nucleic Acids
- Coil-Helix Transition
- 3D Structure of Proteins & Nucleic Acids
- Protein Function: Energetics of Enzyme Catalysis
- Protein Function: Energetics of Membrane Transport Processes
- Protein Folding Models
- Thermodynamics of Protein Folding
- Kinetics of Protein Folding
- Interactions Between Macromolecules
- Non-Cooperative Macromolecule-Ligand Interactions
- Cooperative Macromolecule-Ligand Interactions
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| Capaian Pembelajaran Mata Kuliah (CPMK) | - Menjelaskan hukum termodinamika, energi Gibbs, fungsi partisi, dan distribusi Boltzmann serta relevansinya dalam sistem biologis.
- Menjelaskan peran interaksi lemah (ikatan hidrogen, hidrofobik, van der Waals, elektrostatik) dalam stabilitas biomolekul.
- Menganalisis ruang konformasi protein (grafik Ramachandran) dan dinamika konformasi asam nukleat.
- Menganalisis termodinamika denaturasi dan lipatan protein menggunakan model duakeadaan dan konsep lanskap energi.
- Menghitung dan menginterpretasi parameter kinetika lipatan protein dari data chevron plot.
- Menerapkan persamaan Michaelis-Menten, model inhibisi, dan isoterm pengikatan ligan dalam analisis data.
- Menginterpretasi data dari metode spektroskopi (UV-Vis, fluoresensi, CD) untuk karakterisasi biomolekul.
- Menjelaskan prinsip dan aplikasi kristalografi sinar-X, Cryo-EM, dan spektrometri massa dalam biokimia fisika.
- Merancang proposal mini-riset yang mengintegrasikan konsep dan metode biokimia fisika.
| - Explain the laws of thermodynamics, Gibbs energy, partition functions, and the Boltzmann distribution, as well as their relevance to biological systems.
- Explain the role of weak interactions, including hydrogen bonding, hydrophobic interactions, van der Waals interactions, and electrostatic interactions, in biomolecular stability.
- Analyse protein conformational space using Ramachandran plots and the conformational dynamics of nucleic acids.
- nalyse the thermodynamics of protein denaturation and folding using the two-state model and the concept of the energy landscape.
- Calculate and interpret protein-folding kinetic parameters from chevron plot data.
- Apply the Michaelis–Menten equation, inhibition models, and ligand-binding isotherms in data analysis.
- Interpret data from spectroscopic methods, including UV–Vis, fluorescence, and circular dichroism (CD), for biomolecular characterisation.
- Explain the principles and applications of X-ray crystallography, Cryo-EM, and mass spectrometry in physical biochemistry.
- Design a mini-research proposal that integrates concepts and methods in physical biochemistry.
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